Investigating the effect of ph on amylase activity lab report

Effects of pH on Amylase Activity Essay

investigating the effect of ph on amylase activity lab report

Investigating the effect of pH on amylase activity Repeat the whole procedure with another of the pH buffers or pool your results with others in your class.

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We use cookies to give you the best experience possible. Abstract: Amylase is an important enzyme in the human body as it allows for the consumption of starch by breaking the polysaccharide down into maltose units. All enzymes, including amylase, function best at a certain optimal pH. Therefore, in this experiment, the effect of different pHs on the reaction rate of amylase is studied. It was hypothesized that the amylase-starch reaction would proceed fastest at a pH closest to that of the body, or 7.

Like most proteins, they are synthesized by the ribosomes in the cell. They react with a specific substrate in order to increase the rate of a chemical reaction within the cell. Without enzymes, reactions would be significantly slower and we would not be able to do the most basic functions, such as breathing or digesting food. Amylase is an type of enzyme. It breaks down starch to glucose, giving food that sweet taste. An example of amylase in the natural world is in bananas.

To determine the rate of the amylase activity at different pHs. You will investigate the breakdown of starch by amylase at different pHs. The different pHs under investigation will be produced using buffer solutions. Buffer solutions produce a particular pH, and will maintain it if other substances are added. The amylase will break down the starch. A series of test tubes containing a mixture of starch and amylase is set up at different pHs. A sample is removed from the test tubes every 10 seconds to test for the presence of starch.

Measure the time taken for amylase to completely break down starch , by withdrawing samples at 10 second intervals and noting the time at which the solution no longer gives a blue-black colour with iodine solution but the iodine solution remains orange.
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The Effects of Temperature, pH and Enzyme Concentration on Amylase

The effect of pH on the rate of enzymatic reactions

effect of pH on amylase activity Essay

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Investigating the effect of pH on amylase activity

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5 COMMENTS

  1. Meovematan says:

    Cell metabolism

  2. Grace R. says:

    Nov 24, Investigating the effect of pH on amylase activity groups of five students could complete the investigation by working together and pooling results. Using saliva: the CLEAPSS Laboratory Handbook provides guidance on.

  3. Isabella H. says:

    An enzyme is a type of protein found in all living cells.

  4. Agnès T. says:

    Cell metabolism - Eduqas - Revision 3 - GCSE Biology (Single Science) - BBC Bitesize

  5. Thibaut B. says:

    Accessibility links

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